Brand: Thermo ScientificTM OxoidTM Oxoid Membrane Clostridium Perfringens Agar Base (m-CP Agar Base) is a chromogenic medium for rapid isolation and presumptive identification of Clostridium perfringens from water samples. Also available: Membrane Clostridium Perfringens Selective Supplement, Part No. SR0188E. Membrane Clostridium Perfringens (M-CP) Agar Base, Oxoid Composition Typical Formula * gm/litre Tryptose 30.0 Yeast extract 20.0 Sucrose 5.0 L-cysteine hydrochloride 1.0 Magnesium sulphate.7H2O 0.1 Agar 15.0 Bromocresol purple 0.04 pH 7.6 ± 0.2 @ 25°C (M-CP) Agar Base, Oxoid Preparation: Suspend 35.55g of m-CP Agar Base in 500ml of distilled water Mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of m-CP Selective Supplement reconstituted as directed. Aseptically add the following sterile solutions dissolved in distilled water: Component Solution Strength Volume (ml) Phenolphthalein biphosphate tetrasodium salt 0.5% 10.0 Ferric chloride hexahydrate 4.5% 1.0 Indoxyl b-D-glucoside 0.75% 4.0 Storage conditions and Shelf life Store the dehydrated medium at 10-30°C and use before the expiry date on the label. Prepared medium may be stored for up to 5 days at 2-8°C in the dark.
