Brand: Thermo ScientificTM OxoidTM Oxoid Membrane Clostridium Perfringens Selective Supplement (m-CP) is a chromogenic medium for rapid and presumptive identification of Clostridium perfringens from water samples. Add to Membrane Clostridium Perfringens (m-CP) Agar Base, Part No. CM0992B Each vial of SR0188E supplements 500mL of medium 1M-Cp Selective Supplement, Oxoid Composition Vial contents (each vial is sufficient for 500ml of medium) per vial per litre Polymyxin B sulphate 12.5mg (105,000IU) 25.0mg (210,000IU) D-Cycloserine 200.0mg 400.0mg 1M-Cp Selective Supplement, Oxoid Preparation: Suspend 35.55g of m-CP Agar Base in 500ml of distilled water Mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of m-CP Selective Supplement reconstituted as directed. Aseptically add the following sterile solutions dissolved in distilled water: Component Solution Strength Volume (ml) Phenolphthalein biphosphate tetrasodium salt 0.5% 10.0 Ferric chloride hexahydrate 4.5% 1.0 Indoxyl b-D-glucoside 0.75% 4.0 Storage conditions and Shelf life m-CP Selective Supplement SR0188 should be stored at 2-8°C. Store the prepared medium at 2-8°C.
