Brand: Thermo ScientificTM OxoidTM Oxoid Aeromonas Medium Base (Ryan) is a selective differential medium for the isolation of Aeromonas hydrophila. Also available Ampicillin Selective Supplement, Part No. SR0136E. Aeromonas Medium Base (Ryan), Oxoid Composition Typical Formula * gm/litre Proteose peptone 5.0 Yeast extract 3.0 L. Lysine monohydrochloride 3.5 L. Arginine monohydrochloride 2.0 Sorbitol 3.0 Inositol 2.5 Lactose 1.5 Xylose 3.75 Bile Salts No.3 3.0 Sodium thiosulphate 10.67 Sodium chloride 5.0 Ferric ammonium citrate 0.8 Bromothymol blue 0.04 Thymol blue 0.04 Agar 12.5 pH 8.0 ± 0.2 @ 25°C Aeromonas Medium Base (Ryan), Oxoid Preparation: Suspend 29.5g in 500ml of distilled water. Bring gently to the boil. DO NOT AUTOCLAVE. Cool to 50°C and aseptically add one vial of Ampicillin Selective Supplement SR0136 reconsituted as directed. Mix well and pour plates. Storage conditions and Shelf life Store the dehydrated medium at 10-30°C and use before the expiry date on the label. Store the prepared plates of medium 2-8°C. Precautions Although Aeromonas and Plesiomonas spp will grow on the medium if ampicillin is omitted, it will be more difficult to distinguish them from the other organisms present on the plate. Suspected colonies of Aeromonas spp must be confirmed by biochemical tests.
