Brand: Thermo ScientificTM OxoidTM Oxoid AFPA Medium Base is a selective identification medium for the detection of Aspergillus flavus and Aspergillus parasiticus. Also available: Membrane Clostridium Perfringens Selective Supplement, Part No. SR0188E. AFPA Medium Base, Oxoid Composition Typical Formula * gm/litre Peptone 10.0 Yeast extract 20.0 Ferric ammonium Citrate 0.5 Dichloran 0.002 Agar 15.0 pH 6.3 ± 0.2 @ 25°C AFPA Medium Base, Oxoid Preparation: Suspend 22.75g in 500ml (45.5g/l) of distilled water and heat to dissolve completely. Reconstitute one vial SR0078E per 500ml medium or one vial SR0078H per 2 litres medium, as directed. Add the vial contents to the AFPA Base. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Mix well and pour into sterile Petri dishes. Storage conditions and Shelf life Store the dehydrated medium at 10-30°C and use before the expiry date on the label. Store the prepared plates at 2-8°C.
